RT Journal Article
T1 Eco-friendly extraction of Mastocarpus stellatus carrageenan for the synthesis of gold nanoparticles with improved biological activity
A1 González Ballesteros, Noelia
A1 Torres Pérez, María Dolores
A1 Flórez Fernández, Noélia
A1 Diego González, Lara
A1 Simón Vázquez, Rosana
A1 Rodríguez Argüelles, Maria Carmen
A1 Domínguez González, Herminia
K1 2303 Química Inorgánica
AB Carrageenan was extracted from Mastocarpus stellatus using hot water extraction under atmospheric and pressurized conditions. The influence of heating temperature during a non-isothermal heating profile up to temperatures in the range 70–190 °C was studied to evaluate the extraction yields and properties of the carrageenan fraction. Under the selected conditions (130 °C), extracted carrageenan (CMs) was used for the green synthesis of gold nanoparticles (AuNPs). After the optimization of the reaction conditions, the synthesized gold nanoparticles (Au@CMs) were characterized by UV–Vis spectroscopy, Z potential measurements, electron microscopy, and X-ray diffraction analysis, which confirmed the formation of spherical, polycrystalline, and negatively charged nanoparticles with a mean diameter of 14.3 ± 2.1 nm. The study conducted by scanning transmission electron microscopy, energy dispersive X-ray analysis and mapping confirmed the presence of carrageenan stabilizing AuNPs. Finally, Fourier transformed infrared spectroscopy was performed to analyze the functional groups of CMs involved in the reduction and stabilization of AuNPs. The selective cytotoxicity and the antioxidant activity of the Au@CMs were evaluated in different cell lines and compared to the CMs. Au@CMs showed an improved antioxidant capacity in cells under oxidative stress and the induction of apoptosis in a monocytic cell line, while no antitumor effect was observed in a lung endothelial cell line.
PB International Journal of Biological Macromolecules
SN 01418130
YR 2021
FD 2021-07-31
LK http://hdl.handle.net/11093/8476
UL http://hdl.handle.net/11093/8476
LA eng
NO International Journal of Biological Macromolecules, 183, 1436-1449 (2021)
NO Agencia Estatal de Investigación | Ref. RTI2018-096376-B-I00
DS Investigo
RD 25-abr-2025