RT Journal Article
T1 Au@Ag core–shell nanoparticles for colorimetric and surface-enhanced Raman-scattering-based multiplex competitive lateral flow immunoassay for the simultaneous detection of histamine and parvalbumin in fish
A1 Fernandez Lodeiro, Carlos
A1 Gonzalez Cabaleiro, Lara
A1 Vázquez Iglesias, Lorena
A1 Serrano Pertierra, Esther
A1 Bodelón González, Gustavo
A1 Carrera Mouriño, Mónica
A1 Blanco López, María Carmen
A1 Pérez Juste, Jorge
A1 Pastoriza Santos, Isabel
K1 2301 Química Analítica
AB Foodborne allergies and illnesses represent a major global health concern. In particular, fish can trigger life-threatening food allergic reactions and poisoning effects, mainly caused by the ingestion of parvalbumin toxin. Additionally, preformed histamine in less-than-fresh fish serves as a toxicological alert. Consequently, the analytical assessment of parvalbumin and histamine levels in fish becomes a critical public health safety measure. The multiplex detection of both analytes has emerged as an important issue. The analytical detection of parvalbumin and histamine requires different assays; while the determination of parvalbumin is commonly carried out by enzyme-linked immunosorbent assay, histamine is analyzed by high-performance liquid chromatography. In this study, we present an approach for multiplexing detection and quantification of trace amounts of parvalbumin and histamine in canned fish. This is achieved through a colorimetric and surface-enhanced Raman-scattering-based competitive lateral flow assay (SERS-LFIA) employing plasmonic nanoparticles. Two distinct SERS nanotags tailored for histamine or β-parvalbumin detection were synthesized. Initially, spherical 50 nm Au@Ag core–shell nanoparticles (Au@Ag NPs) were encoded with either rhodamine B isothiocyanate (RBITC) or malachite green isothiocyanate (MGITC). Subsequently, these nanoparticles were bioconjugated with anti-β-parvalbumin and antihistamine, forming the basis for our detection and quantification methodology. Additionally, our approach demonstrates the use of SERS-LFIA for the sensitive and multiplexed detection of parvalbumin and histamine on a single test line, paving the way for on-site detection employing portable Raman instruments.
PB ACS Applied Nanomaterials
SN 25740970
YR 2023
FD 2023-12-19
LK http://hdl.handle.net/11093/7553
UL http://hdl.handle.net/11093/7553
LA eng
NO ACS Applied Nanomaterials, 7(1): 498-508 (2024)
NO Agencia Estatal de Investigación | Ref. PID2019-108954RB-I00
DS Investigo
RD 13-ene-2025