RT Journal Article
T1 Design of green DES-based aqueous two phase systems for lipolytic enzymes extraction
A1 Álvarez Álvarez, María Salomé
A1 Longo González, María Asunción
A1 Deive Herva, Francisco Javier
A1 Rodríguez Rodríguez, Ana María
K1 3303 Ingeniería y Tecnología Químicas
AB This study pioneers a novel green and cost-effective separation process using reline deep eutectic solvent (DES) for extracting Thermomyces lanuginosus lipase (TlL) from aqueous solutions. For the first time, it has been shown that various potassium organic salts combined with reline DES can induce phase separation in aqueous solutions of the biodegradable non-ionic surfactant Tergitol 15-S9, presenting a groundbreaking alternative to the banned surfactant Triton X-100. This work uniquely applies three different equations to describe the experimental solubility curves. The selection of reline DES is particularly innovative as it avoids significant deactivation effects with three commercial lipases: Candida antarctica lipases A and B, and TlL, with the latter undergoing a detailed study. This includes an unprecedented analysis of its thermodeactivation kinetics in reline DES aqueous solutions. Additionally, a novel investigation of immiscibility regions at different temperatures, involving comprehensive Tie-Line characterizations (slope and length), preceded the evaluation of lipase extraction. Remarkably, the study demonstrates that optimizing the feed composition to contain higher water concentrations can achieve exceptionally high extraction efficiencies (approximately 90%), suggesting a transformative potential for future applications in real aqueous cultures
PB Journal of Molecular Liquids
SN 01677322
YR 2024
FD 2024-11-15
LK http://hdl.handle.net/11093/7544
UL http://hdl.handle.net/11093/7544
LA eng
NO Journal of Molecular Liquids, 414, 126075 (2024)
NO Universidade de Vigo/CISUG
DS Investigo
RD 20-ene-2025