RT Journal Article T1 Isolation of novel benzo[a]anthracene-degrading microorganisms and continuous bioremediation in an expanded-bed bioreactor A1 Rosales Villanueva, Emilio A1 Pérez Paz, Alicia A1 Vázquez, X. A1 Pazos Currás, Marta María A1 Sanromán Braga, María Ángeles K1 3303 Ingeniería y Tecnología Químicas K1 3302.90 Ingeniería Bioquímica AB In the present work, several samples from labwaste containers polluted with polycyclic aromatic hydrocarbons (PAHs) and heavy metals were investigated as potentialsources of PAH-degrading microorganisms. After isolating,two fungal strains were selected as the best degrading microorganisms. Genetic identification by sequencing was carriedout and they were identified as Trichoderma longibrachiatumand Byssochlamys spectabilis. Their degradation ability wasdetermined in liquid cultures with 100 lM of benzo[a]anthracene. T. longibrachiatum cultures showed highest degradation values (around 97%) after 9 days, furthermore in asecond batch the time was reduced to 6 days. To analyse theviability of industrial application, a continuous treatment in anexpanded-bed bioreactor was developed operating at differentresidence times with T. longibrachiatumimmobilised on cubesof nylon sponge. It is noticeable that the bioreactor working incontinuous mode was able to operate without operationalproblems and attaining high degradation levels depending onthe residence time PB Bioprocess and Biosystems Engineering SN 16157591 YR 2011 FD 2011-12-15 LK http://hdl.handle.net/11093/6914 UL http://hdl.handle.net/11093/6914 LA eng NO Bioprocess and Biosystems Engineering, 35(5): 851-855 (2012) NO Xunta de Galicia | Ref. 08MDS034314PR DS Investigo RD 16-feb-2025