RT Journal Article T1 Radiotherapy metastatic prostate cancer cell lines treated with gold nanorods modulate miRNA signatures A1 Carneiro Soares, Sílvia A1 Aires, Fátima A1 Monteiro, Armanda A1 Pinto, Gabriela A1 Faria, Isabel A1 Sales, Goreti A1 Correa Duarte, Miguel Ángel A1 Guerreiro, Susana A1 Fernandes, Rúben K1 3201.12 Radioterapia AB MicroRNA (miRNA) modulation has been identified as a promising strategy for improving the response of human prostate cancer (PCa) to radiotherapy (RT). Studies have shown that mimics or inhibitors of miRNAs could modulate the sensitivity of PCa cells to RT. In addition, pegylated gold nanoparticles have been studied as a therapeutic approach to treat PCa cells and/or vehicles for carrying miRNAs to the inside of cells. Therefore, we evaluated the capacity of hypofractionated RT and pegylated gold nanorods (AuNPr-PEG) to modulate the miRNA signature on PCa cells. Thus, RT-qPCR was used to analyze miRNA-95, miRNA-106-5p, miRNA-145-5p, and miRNA-541-3p on three human metastatic prostate cell lines (PC3, DU145, and LNCaP) and one human prostate epithelial cell line (HprEpiC, a non-tumor cell line) with and without treatment. Our results showed that miRNA expression levels depend on cell type and the treatment combination applied using RT and AuNPr-PEG. In addition, cells pre-treated with AuNPr-PEG and submitted to 2.5 Gy per day for 3 days decreased the expression levels of miRNA-95, miRNA-106, miRNA-145, and miRNA-541-3p. In conclusion, PCa patients submitted to hypofractionated RT could receive personalized treatment based on their metastatic cellular miRNA signature, and AuNPr-PEG could be used to increase metastatic cell radiosensitivity. PB International Journal of Molecular Sciences SN 14220067 YR 2024 FD 2024-02-27 LK http://hdl.handle.net/11093/6526 UL http://hdl.handle.net/11093/6526 LA eng NO International Journal of Molecular Sciences, 25(5): 2754 (2024) NO Fundação para a Ciência e a Tecnologia | Ref. 2022.09032.PTDC DS Investigo RD 13-dic-2024