RT Journal Article T1 Tetraconazole alters the methionine and ergosterol biosynthesis pathways in Saccharomyces yeasts promoting changes on volatile derived compounds A1 Sieiro Sampedro, Thais A1 Briz Cid, Noelia A1 Pose Juan, Eva A1 Figueiredo González, María A1 González Barreiro, Carmen A1 Simal Gándara, Jesús A1 Cancho Grande, Beatriz A1 Rial Otero, Raquel K1 3309.29 Vino K1 3309.92 Bioquímica y Microbiología de Los Procesos Fermentativos K1 3309.10 Aroma y Sabor AB The influence of antifungal tetraconazole residues (either as an active substance or as a commercial formulation product) on the fermentative activity of Saccharomyces cerevisiae yeast was evaluated in pasteurized Garnacha red must by using laboratory-scale fermentation assays. The presence of this fungicide promoted a slight decrease in glucose consumption. Volatile fermentative-derived compounds were evaluated in deep. Statistically significant changes were found in methionol (with a mean decrease of around 24%), fatty acids (with increments ranged from 23% to 66%), and ethyl esters (with increases ranged from 23% to 145%) contents when grape musts were enriched with the commercial formulation at both contamination levels assayed. Based on protein mass fingerprinting analysis, it was possible to relate these variations on volatiles content with changes in the activity of several enzymes (Met3p, Met14p, Adh2p, Hmg1p, Erg5p, Erg6p, Erg11p, and Erg20p) involved in the secondary metabolism of yeasts. PB Food Research International SN 09639969 YR 2020 FD 2020-04 LK http://hdl.handle.net/11093/6179 UL http://hdl.handle.net/11093/6179 LA eng NO Food Research International, 130, 108930 (2020) NO Ministerio de Economía y Competitividad | Ref. AGL2015-66491-C2-1-R DS Investigo RD 23-ene-2025