RT Journal Article
T1 Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions
A1 Gonzalez Cabaleiro, Laura
A1 Álvarez Álvarez, María Salomé
A1 Alonso Rodríguez, Ana María
A1 Longo González, María Asunción
A1 Deive Herva, Francisco Javier
K1 3303.03 Procesos Químicos
AB The ongoing energy crisis has spurred increased research into sustainable and more competitive methods for producing biofuels, including biodiesel. In this context, the focus of the current study is to underscore the viability of investing in a novel biocatalytic complex. This complex incorporates extremophilic lipases and biocompatible ionic liquids with the aim of achieving exceptionally high conversions in transesterification reactions without generating glycerol. Through a meticulous screening process encompassing various amino acid and dipeptide-based ionic liquids from the ammonium family, cholinium glycinate turned out to be the optimal choice. This selection was driven not only by its enhanced compatibility with a commercially available Candidaantarctica lipase B (CaLB) but also with extremophilic enzymes synthesized in-house, derived from halophilic(Halomonas spLM1C) and thermophilic (Thermus thermophilus HB27) strains. Following rigorous testing of both free and immobilized enzymes, the ideal concentration of the ionic liquid in transesterification reactions was determined to be 1% relative to the sunflower oil content. Comparative analysis of conversion rates between immobilized thermophilic lipase and immobilized CaLB revealed the efficacy of the proposed approach. Maximum conversions were found to increase by 20%, with specific conversion rates soaring by approximately 180% when utilizing the immobilized thermophilic lipase. In conclusion, this research ushers in new prospects for advancing the competitiveness of biocatalytic solutions in glycerol-free transesterification reactions, underscoring its potential to revolutionize the landscape of sustainable energy production.
PB Energy Conversion and Management: X
SN 25901745
YR 2023
FD 2023-10
LK http://hdl.handle.net/11093/5261
UL http://hdl.handle.net/11093/5261
LA eng
NO Energy Conversion and Management: X, 20: 100456 (2023)
NO Agencia Estatal de Investigación | Ref. RTI2018-094702-B-I00
DS Investigo
RD 03-dic-2024