RT Journal Article
T1 Exploring the interplay between metabolic pathways and taxane production in elicited Taxus baccata cell suspensions
A1 Pérez Matas, Edgar
A1 García Pérez, Pascual
A1 Miras Moreno, Begoña
A1 Lucini, Luigi
A1 Bonfill, Mercedes
A1 Palazon, Javier
A1 Hidalgo Martínez, Diego
K1 2417.19 Fisiología Vegetal
AB Taxus cell cultures are a reliable biotechnological source of the anticancer drug paclitaxel. However, the interplay between taxane production and other metabolic pathways during elicitation remains poorly understood. In this study, we combined untargeted metabolomics and elicited Taxus baccata cell cultures to investigate variations in taxane-associated metabolism under the influence of 1 µM coronatine (COR) and 150 µM salicylic acid (SA). Our results demonstrated pleiotropic effects induced by both COR and SA elicitors, leading to differential changes in cell growth, taxane content, and secondary metabolism. Metabolite annotation revealed significant effects on N-containing compounds, phenylpropanoids, and terpenoids. Multivariate analysis showed that the metabolomic profiles of control and COR-treated samples are closer to each other than to SA-elicited samples at different time points (8, 16, and 24 days). The highest level of paclitaxel content was detected on day 8 under SA elicitation, exhibiting a negative correlation with the biomarkers kauralexin A2 and taxusin. Our study provides valuable insights into the intricate metabolic changes associated with paclitaxel production, aiding its potential optimization through untargeted metabolomics and an evaluation of COR/SA elicitor effects.
PB Plants
SN 22237747
YR 2023
FD 2023-07-19
LK http://hdl.handle.net/11093/5180
UL http://hdl.handle.net/11093/5180
LA eng
NO Plants, 12(14): 2696 (2023)
NO Agencia Estatal de Investigación | Ref. PID2020-113438RB-I00
DS Investigo
RD 14-ene-2025