RT Journal Article
T1 Impact of elicitation on plant antioxidants production in Taxus cell cultures
A1 Pérez Matas, Edgar
A1 García Pérez, Pascual
A1 Bonfill, Mercedes
A1 Lucini, Luigi
A1 Hidalgo Martínez, Diego
A1 Palazon, Javier
K1 2301 Química Analítica
AB Elicited cell cultures of Taxus spp. are successfully used as sustainable biotechnological production systems of the anticancer drug paclitaxel, but the effect of the induced metabolomic changes on the synthesis of other bioactive compounds by elicitation has been scarcely studied. In this work, a powerful combinatorial approach based on elicitation and untargeted metabolomics was applied to unravel and characterize the effects of the elicitors 1 µM of coronatine (COR) or 150 µM of salicylic acid (SA) on phenolic biosynthesis in Taxus baccata cell suspensions. Differential effects on cell growth and the phenylpropanoid biosynthetic pathway were observed. Untargeted metabolomics analysis revealed a total of 83 phenolic compounds, mainly flavonoids, phenolic acids, lignans, and stilbenes. The application of multivariate statistics identified the metabolite markers attributed to elicitation over time: up to 34 compounds at 8 days, 41 for 16 days, and 36 after 24 days of culture. The most notable metabolic changes in phenolic metabolism occurred after 8 days of COR and 16 days of SA elicitation. Besides demonstrating the significant and differential impact of elicitation treatments on the metabolic fingerprint of T. baccata cell suspensions, the results indicate that Taxus ssp. biofactories may potentially supply not only taxanes but also valuable phenolic antioxidants, in an efficient optimization of resources.
PB Antioxidants
SN 20763921
YR 2023
FD 2023-04-05
LK http://hdl.handle.net/11093/5152
UL http://hdl.handle.net/11093/5152
LA eng
NO Antioxidants, 12(4): 887 (2023)
NO Agencia Estatal de Investigación | Ref. PID2020-113438RB-I00
DS Investigo
RD 25-ene-2025