RT Journal Article
T1 Valorization of Punica granatum L. leaves extracts as a source of bioactive molecules
A1 Gonçalves Marcelino, Sandra
A1 Mandim, Filipa
A1 Oludemi , Taofiq Ayodele
A1 São Pedro Pires, Tânia Cristina
A1 Finimundy, Tiane C.
A1 Prieto Lage, Miguel Ángel
A1 Barros, Lillian
K1 2301.03 Análisis Cromatográfico
AB Due to a lack of innovative valorization strategies, pomegranate processing generates a significant amount of residues with a negative environmental footprint. These by-products are a rich source of bioactive compounds with functional and medicinal benefits. This study reports the valorization of pomegranate leaves as a source of bioactive ingredients using maceration, ultrasound, and microwave-assisted extraction techniques. The phenolic composition of the leaf extracts was analyzed using an HPLC-DAD-ESI/MSn system. The extracts’ antioxidant, antimicrobial, cytotoxic, anti-inflammatory, and skin-beneficial properties were determined using validated in vitro methodologies. The results showed that gallic acid, (-)-epicatechin, and granatin B were the most abundant compounds in the three hydroethanolic extracts (between 0.95 and 1.45, 0.7 and 2.4, and 0.133 and 3.0 mg/g, respectively). The leaf extracts revealed broad-spectrum antimicrobial effects against clinical and food pathogens. They also presented antioxidant potential and cytotoxic effects against all tested cancer cell lines. In addition, tyrosinase activity was also verified. The tested concentrations (50–400 µg/mL) ensured a cellular viability higher than 70% in both keratinocyte and fibroblast skin cell lines. The obtained results indicate that the pomegranate leaves could be used as a low-cost source of value-added functional ingredients for potential nutraceutical and cosmeceutical applications.
PB Pharmaceuticals
SN 14248247
YR 2023
FD 2023-02-23
LK http://hdl.handle.net/11093/5146
UL http://hdl.handle.net/11093/5146
LA eng
NO Pharmaceuticals, 16(3): 342 (2023)
NO Ministerio de Ciencia e Innovación | Ref. RYC-2017-22891
DS Investigo
RD 11-dic-2024