RT Dissertation/Thesis T1 Characterization of the main factors controlling Boletus edulis and Boletus reticulatus mycorrhization on chestnut hybrids (Castanea × coudercii) T2 Caracterización dos principais factores que controlan a micorrización de Boletus edulis e Boletus reticulatus en híbridos de castiñeiros (Castanea × coudercii) A1 Iglesias Bernabé, Laura K1 2414.06 Hongos K1 3107.04 Fruticultura K1 2511.02 Biología de Suelos AB This Doctoral Dissertation is the result of a R & D contract of collaboration between the company Hifas Foresta S.L. and the Agrobiotech for Health Research Group of the University of Vigo. Hifas Foresta SL has a wide variety of Castanea × coudercii (Castanea sativa x Castanea crenata) hybrids resistant to ink on the market. The use of ectomycorrhizal fungi increases resistance to pathogens, and some, such as Boletus edulis, are of great commercial interest for producing edible mushrooms of high culinary value. That is why this company is especially interested in optimizing the production of mycorrhized chestnut with B. edulis. This optimization will be possible if, in addition to ensuring the formation of ectomycorrhizae, its autophenology is better known to maintain mycorrhization in the field and also induce its fruiting.For this reason, the first objective of this thesis will be to identify the biotic and abiotic factors that affect the mycorrhization of Castanea × coudercii with B. edulis and B. reticulatus under natural conditions. Next, the second objective of this thesis will be to identify the factors that affect the mycorrhization of Castanea × coudercii with B. edulis under artificial or controlled conditions (in vitro). To this end, in vitro culture protocols will be designed for the fungi, helper bacteria and plantlets and formation of ectomycorrhizae in Castanea × coudercii. LK http://hdl.handle.net/11093/4868 UL http://hdl.handle.net/11093/4868 LA eng NO Ministerio de Ciencia, Innovación y Universidades | Ref. RTI2018-095568-BI00 DS Investigo RD 24-mar-2025