RT Journal Article
T1 Fucoidan from Fucus vesiculosus: evaluation of the impact of the sulphate content on nanoparticle production and cell toxicity
A1 Flórez Fernández, Noelia
A1 Pontes, Jorge F.
A1 Guerreiro, Filipa
A1 Afonso, Inês T.
A1 Lollo, Giovanna
A1 Torres Pérez, María Dolores
A1 Domínguez González, Herminia
A1 Costa, Ana M. Rosa da
A1 Grenha, Ana Margarida Moutinho
K1 3303.03 Procesos Químicos
K1 2417.07 Algología (Ficología)
K1 3390.01 Biotecnología de Microalgas
AB The composition of seaweeds is complex, with vitamins, phenolic compounds, minerals, and polysaccharides being some of the factions comprising their structure. The main polysaccharide in brown seaweeds is fucoidan, and several biological activities have been associated with its structure. Chitosan is another marine biopolymer that is very popular in the biomedical field, owing to its suitable features for formulating drug delivery systems and, particularly, particulate systems. In this work, the ability of fucoidan to produce nanoparticles was evaluated, testing different amounts of a polymer and using chitosan as a counterion. Nanoparticles of 200–300 nm were obtained when fucoidan prevailed in the formulation, which also resulted in negatively charged nanoparticles. Adjusting the pH of the reaction media to 4 did not affect the physicochemical characteristics of the nanoparticles. The IC50 of fucoidan was determined, in both HCT−116 and A549 cells, to be around 160 µg/mL, whereas it raised to 675–100 µg/mL when nanoparticles (fucoidan/chitosan = 2/1, w/w) were tested. These marine materials (fucoidan and chitosan) provided features suitable to formulate polymeric nanoparticles to use in biomedical applications.
PB Marine Drugs
SN 16603397
YR 2023
FD 2023-02-07
LK http://hdl.handle.net/11093/4655
UL http://hdl.handle.net/11093/4655
LA eng
NO Marine Drugs, 21(2): 115 (2023)
NO Fundação para a Ciência e a Tecnologia | Ref. UIDB/04326/2020
DS Investigo
RD 25-ene-2025