RT Journal Article T1 Optimization of the use of cellulolytic enzyme preparation for the extraction of health promoting anthocyanins from black carrot using response surface methodology A1 Kumar, Manoj A1 Dahuja, Anil A1 Sachdev, Archana A1 Tomar, Maharishi A1 Lorenzo Rodríguez, José Manuel A1 Dhumal, Sangram A1 Radha A1 Chandran, Deepak A1 Varghese, Eldho A1 Saha, Supradip A1 Sairam, K.V.S.S. A1 Singh, Surinder A1 Senapathy, Marisennayya A1 Amarowicz, Ryszard A1 Kaur, Charanjit A1 Kennedy, John F. A1 Mekhemar, Mohamed K1 3309 Tecnología de Los Alimentos K1 3309.08 Colorantes AB Anthocyanin-rich extracts from black carrots are being considered as a candidate replacer for the red colour in processed foods. The present investigation optimizes the extraction of anthocyanins with high phenolic contentand low degradation parameters from black carrots using a cellulolytic multi-enzyme preparation known as Viscozyme. The optimized conditions for Viscozyme using a Box-Behnken design (BBD) of response surface methodology (RSM) were as follow: temperature (50.2 ◦C), extraction time (58.4 min) and enzyme concentration(0.20%). The predicted value of anthocyanins content was 1380 mg/L, which was near to the optimizedexperimental value of 1375 mg/L. The extracted anthocyanins based on above mentioned conditions exhibitedthe lowest degradation parameters such as degradation index (DI) of (0.86), browning index of (BI) (1.31) andwere characterized with cyanidin 3-sinapoylxylosylglucosylgalactoside as being the most abundant. The findingsclearly reveal that Viscozyme-assisted extraction (VAE) is the best approach for extracting superior quality extractsfrom black carrots with high anthocyanin and other phenolic component concentrations. PB LWT - Food Science and Technology SN 00236438 YR 2022 FD 2022-06 LK http://hdl.handle.net/11093/4577 UL http://hdl.handle.net/11093/4577 LA eng NO LWT - Food Science and Technology, 163, 113528 (2022) DS Investigo RD 09-dic-2024