RT Journal Article
T1 Evaluating the alterations of the estrogen-responsive genes in Cyprinodon variegatus larvae for biomonitoring the impacts of estrogenic endocrine disruptors (EEDs)
A1 Moreno Abril, Sandra Isabel
A1 Olmos Pin, Ana
A1 MARTINEZ SCHöNEMANN, Alexandre
A1 Bellot, Marina
A1 Gómez Canela, Cristian
A1 Beiras Garcia Sabell, Ricardo
K1 2499 Otras Especialidades Biológicas
AB Currently, endocrine disruptors (EDs) can be found in all the environmental compartments. To understand the effects of estrogenic EDs (EEDs), adults of Cyprinodon variegatus have been classically used as a marine model. However, it is during development that exposure to contaminants may generate permanent consequences. Thus, the aim of this study was to verify the effects produced by acute exposure to 17α-ethinylestradiol (EE2) in C. variegatus larvae. Quantitative PCR (qPCR) results revealed the induction of vtg and zp gene expression on exposure to 1000 ng/L EE2 and the induction of vtgc, zp2, zp3 and cyp19a2, and inhibition of vtgab, wap and cyp1a1 on exposure to 100 ng/L EE2. Lower concentrations inhibited the gene expression of vtgab and wap (50 ng/L), cyp1a1 (25 ng/L) and zp2 (12.5 ng/L). These alterations in gene expression allow us to affirm that larvae of C. variegatus are an efficient and sensitive model for biomonitoring EEDs.
PB Environmental Toxicology and Pharmacology
SN 13826689
YR 2023
FD 2023-01
LK http://hdl.handle.net/11093/4353
UL http://hdl.handle.net/11093/4353
LA eng
NO Environmental Toxicology and Pharmacology, 97: 104042 (2023)
NO Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG
DS Investigo
RD 11-dic-2024