RT Journal Article
T1 Evolution shapes the responsiveness of the D-box enhancer element to light and reactive oxygen species in vertebrates
A1 Pagano, Cristina
A1 Siauciunaite, Rima
A1 Idda, María L.
A1 Ruggiero, Gennaro
A1 Ceinos Caride, Rosa María
A1 Pagano, Martina
A1 Frigato, Elena
A1 Bertolucci, Cristiano
A1 Foulkes, Nicholas S
A1 Vallone, Daniela
K1 2401.13 Fisiología Animal
K1 3105 Peces y Fauna Silvestre
K1 2411 Fisiología Humana
AB The circadian clock is a highly conserved cell-autonomous mechanism that directs daily rhythms in most aspects of biology. Daily entrainment by environmental signals, notably light, is essential for its function. However, our understanding of the mechanisms and the evolution of photic entrainment remains incomplete. Fish represent attractive models for exploring how light regulates the circadian clock due to the direct light sensitivity of their peripheral clocks. Central to this property is the light induced expression of clock genes that is mediated by D-box enhancer elements. Here, using zebrafish cells, we reveal that the light responsive D-box enhancer serves as a nuclear target for reactive oxygen species (ROS). We demonstrate that exposure to short wavelengths of visible light triggers increases in ROS levels via NADPH oxidase activity. Elevated ROS activates the JNK and p38 MAP kinases and in turn, induces clock gene expression via the D-box. In blind cavefish and mammals, where peripheral clocks are no longer entrained by direct illumination, ROS levels are still increased upon light exposure. However, in these species ROS no longer induces D-box driven clock gene transcription. Thus, during evolution, alterations in ROS-responsive signal transduction pathways underlie fundamental changes in peripheral clock photoentrainment.
PB Scientific Reports
SN 20452322
YR 2018
FD 2018-09-04
LK http://hdl.handle.net/11093/4323
UL http://hdl.handle.net/11093/4323
LA eng
NO Scientific Reports, 8(1): 13180 (2018)
NO Universidad de Ferrara | Ref. FAR2014–2017
DS Investigo
RD 08-feb-2025