RT Journal Article
T1 Genome-wide association analysis for maize stem Cell Wall-bound Hydroxycinnamates
A1 López Malvar, Ana
A1 Butrón Gómez, Ana María
A1 Samayoa, Luis Fernando
A1 Figueroa Garrido, David Jose
A1 Malvar Pintos, Rosa Ana
A1 Santiago Carabelos, Rogelio
K1 3103.04 Protección de Los Cultivos
K1 2417.09 Fitopatología
K1 2417.14 Genética Vegetal
AB Background: The structural reinforcement of cell walls by hydroxycinnamates has a significant role in defense againstpests and pathogens, but it also interferes with forage digestibility and biofuel production. Elucidation of maize geneticvariations that contribute to variation for stem hydroxycinnamate content could simplify breeding for cell wallstrengthening by using markers linked to the most favorable genetic variants in marker-assisted selection orgenomic selection approaches.Results: A genome-wide association study was conducted using a subset of 282 inbred lines from a maizediversity panel to identify single nucleotide polymorphisms (SNPs) associated with stem cell wall hydroxycinnamatecontent. A total of 5, 8, and 2 SNPs were identified as significantly associated to p-coumarate, ferulate, and totaldiferulate concentrations, respectively in the maize pith. Attending to particular diferulate isomers, 3, 6, 1 and 2 SNPswere related to 8–O–4 diferulate, 5–5 diferulate, 8–5 diferulate and 8–5 linear diferulate contents, respectively. Thisstudy has the advantage of being done with direct biochemical determinations instead of using estimates based onNear-infrared spectroscopy (NIRS) predictions. In addition, novel genomic regions involved in hydroxycinnamatecontent were found, such as those in bins 1.06 (for FA), 4.01 (for PCA and FA), 5.04 (for FA), 8.05 (for PCA), and 10.03and 3.06 (for DFAT and some dimers).Conclusions: The effect of individual SNPs significantly associated with stem hydroxycinnamate content was low,explaining a low percentage of total phenotypic variability (7 to 10%). Nevertheless, we spotlighted new genomic regionsassociated with the accumulation of cell-wall-bound hydroxycinnamic acids in the maize stem, and genes involved in cellwall modulation in response to biotic and abiotic stresses have been proposed as candidate genes for those quantitativetrait loci (QTL). In addition, we cannot rule out that uncharacterized genes linked to significant SNPs could be implicatedin dimer formation and arobinoxylan feruloylation because genes involved in those processes have been poorlycharacterized. Overall, genomic selection considering markers distributed throughout the whole genome seems to be amore appropriate breeding strategy than marker-assisted selection focused in markers linked to QTL.
PB BMC Plant Biology
SN 14712229
YR 2019
FD 2019-11-27
LK http://hdl.handle.net/11093/4160
UL http://hdl.handle.net/11093/4160
LA eng
NO BMC Plant Biology, 19(1): 519 (2019)
NO Xunta de Galicia | Ref. ED431F 2016/014
DS Investigo
RD 09-dic-2024