RT Journal Article
T1 Biorefinery of brewery spent grain by solid-state fermentation and ionic liquids
A1 Outeiriño Rodriguez, David
A1 Costa Trigo, Iván
A1 Oliveira, Ricardo Pinheiro de Souza
A1 Pérez Guerra, Nelson
A1 Salgado Seara, José Manuel
A1 Domínguez González, José Manuel
K1 3303 Ingeniería y Tecnología Químicas
K1 3308 Ingeniería y Tecnología del Medio Ambiente
K1 2302 Bioquímica
AB Novel environmentally friendly pretreatments have been developed in recent years to improve biomass fractionation. Solid-state fermentation (SSF) and treatment with ionic liquids show low environmental impact and can be used in biorefinery of biomass. In this work, these processes were assessed with brewery spent grain (BSG). First, BSG was used as a substrate to produce cellulases and xylanases by SSF with the fungi Aspergillus brasiliensis CECT 2700 and Trichoderma reesei CECT 2414. Then, BSG was pretreated with the ionic liquid [N1112OH][Gly] and hydrolyzed with the crude enzymatic extracts. Results showed that SSF of BSG with A. brasiliensis achieved the highest enzyme production; meanwhile, the pretreatment with ionic liquids allowed glucan and xylan fractions to increase and reduce the lignin content. In addition, a mixture of the extracts from both fungi in a ratio of 2.5:0.5 Aspergillus/Trichoderma (v/v) efficiently hydrolyzed the BSG previously treated with the ionic liquid [N1112OH][Gly], reaching saccharification percentages of 80.68%, 54.29%, and 19.58% for glucan, xylan, and arabinan, respectively. In conclusion, the results demonstrated that the BSG biorefinery process developed in this work is an effective way to obtain fermentable sugar-containing solutions, which can be used to produce value-added products.
PB Foods
SN 23048158
YR 2022
FD 2022-11-18
LK http://hdl.handle.net/11093/4146
UL http://hdl.handle.net/11093/4146
LA eng
NO Foods, 11(22): 3711 (2022)
NO São Paulo Research Foundation | Ref. 2018/25511-1
DS Investigo
RD 13-dic-2024