RT Journal Article
T1 In vitro study of the therapeutic potential of brown crude Fucoidans in osteoarthritis treatment
A1 Vaamonde García, Carlos
A1 Capelo Mera, Emma
A1 Flórez Fernández, Noelia
A1 Torres Pérez, María Dolores
A1 Rivas Murias, Beatriz
A1 Mejide Faílde, Rosa
A1 Blanco, Francisco J.
A1 Domínguez González, Herminia
K1 3303.03 Procesos Químicos
K1 3205.09 Reumatología
K1 2417.07 Algología (Ficología)
AB Osteoarthritis, one of the most common joint degenerative pathologies, still has no cure, and current treatments, such as nonsteroidal anti-inflammatory drugs, can cause serious adverse effects when taken for a long time. Brown seaweed crude fucoidans are used for the clinical treatment of several pathologies. In this study, the therapeutical potential of these biocompounds was analyzed in primary chondrocytes and the 260TT human chondrocyte cell line. Crude fucoidan from Undaria pinnatifida (Up) and Sargassum muticum (Sm) was obtained by different extraction techniques (microwave-assisted extraction, pressurized hot-water extraction, ultrasound-assisted extraction) and chemically and structurally characterized by Fourier transform infrared spectroscopy, high-performance size-exclusion chromatography, proton nuclear magnetic resonance, and scanning electron microscopy. Once cell viability was confirmed in chondrocytes treated with crude fucoidans, we evaluated their anti-inflammatory effects, observing a significant reduction in IL-6 production stimulated by IL-1β. Findings were confirmed by analysis of IL-6 and IL-8 gene expression, although only fucoidans from Up achieved a statistically significant reduction. Besides this, the antioxidant capacity of crude fucoidans was observed through the upregulation of Nrf-2 levels and the expression of its transcriptional target genes HO-1 and SOD-2, with compounds from Up again showing a more consistent effect. However, no evidence was found that crude fucoidans modulate senescence, as they failed to reduced β-galactosidase activity, cell proliferation, or IL-6 production in chondrocytes stimulated with etoposide. Thus, the findings of this research seem to indicate that the tested crude fucoidans are capable of partially alleviating OA-associated inflammation and oxidative stress, but fail to attenuate chondrocyte senescence.
PB International Journal of Molecular Sciences
SN 14220067
YR 2022
FD 2022-11-17
LK http://hdl.handle.net/11093/4141
UL http://hdl.handle.net/11093/4141
LA eng
NO International Journal of Molecular Sciences, 23(22): 14236 (2022)
NO Axencia Galega de Innovación-Xunta de Galicia | Ref. IN607A 2021/7
DS Investigo
RD 13-ene-2025