RT Journal Article
T1 Extracting extremophilic lipases from aqueous streams by using biocompatible ionic liquids
A1 Gonzalez Cabaleiro, Laura
A1 Longo González, María Asunción
A1 Álvarez Álvarez, María Salomé
A1 Rodríguez Rodríguez, Ana María
A1 Deive Herva, Francisco Javier
K1 3310.05 Ingeniería de Procesos
K1 3303 Ingeniería y Tecnología Químicas
AB In this work, biocompatible ionic liquids based on aminoacids were employed as extractants to separateextremolipases from aqueous streams. First, the influence of aminoacid and dipeptide-based ionic liquids(cholinium glycinate, ChGly, and cholinium glycylglycinate, ChGlygly) on the lipolytic activity of a commerciallipase from Candida antarctica (CaLB) and in-house synthesized extremophilic lipases fromThermus thermophilus HB27 (TtHB27L) and Halomonas sp. LM1C (HL) was investigated. The combinationof thermophilic enzyme with ChGly turned out to be the optimum combination for maximizing the biocatalyticperformance, clearly improving the levels attained when water was exclusively employed assolvent and also surpassing the activity levels provided for the commercial enzyme CaLB. The saltingout capacity of ChGly in aqueous solutions of biodegradable surfactants Tergitol 15S7 and Tergitol15S9 was discussed, recording immiscibility areas almost covering all the ternary diagrams. The aqueousbiphasic systems were experimentally characterized by determining both tie-lines and solubility curvesat several temperatures and the data was modelled with relevant equations like Merchuk, Othmer-Tobiasand Bancroft ones, as they are the most common ones to describe this kind of equilibrium data. So, ChGlywas applied to extract thermophilic and commercial lipases from aqueous solutions at 313.15 K, achievingvery high extraction levels (about 100 %) for TtHB27L, which clearly surpasses the maximum extractionvalues observed for the commercial enzyme (about 80 %). Finally, the process was simulated at realscale through SuperPro Designer v.8.5 for the production of 385 Kg/year of extremolipase
PB Journal of Molecular Liquids
SN 01677322
YR 2022
FD 2022-11
LK http://hdl.handle.net/11093/4130
UL http://hdl.handle.net/11093/4130
LA eng
NO Journal of Molecular Liquids, 366, 120214 (2022)
NO Financiado para publicación en acceso aberto:Universidadede Vigo/CISUG
NO Xunta de Galicia | Ref. ED481D-2019/017
DS Investigo
RD 11-dic-2024