RT Journal Article T1 Assessment of polluted soil remediation using bacterial community tolerance to heavy metals as an indicator A1 Campillo Cora, Claudia A1 Soto Gómez, Diego A1 Arias Estévez, Manuel A1 Fernández Calviño, David K1 2511.04 Química de Suelos K1 2303.18 Metales K1 3308 Ingeniería y Tecnología del Medio Ambiente AB The assessment of remediation on metal-polluted soils is usually focused on total and/or bioavailable metal content. However, these chemical variables do not provide direct information about reductions in heavy metals pressure on soil microorganisms. We propose the use of bacterial communities to evaluate the efficiency of three remediation techniques: crushed mussel shell (CMS) and pine bark (PB) as soil amendments and EDTA-washing. A soil sample was polluted with different doses of Cu, Ni, and Zn (separately). After 30 days of incubation, the remediation techniques were applied, and bacterial community tolerance to heavy metals determined. If bacterial communities develop tolerance, it is an indicator that the metal is exerting toxicity on them. Soil bacterial communities developed tolerance to Cu, Ni, and Zn in response to metal additions. After remediation, bacterial communities showed decreases in bacterial community tolerance to Cu, Ni, and Zn for all remediation techniques. For Cu and Ni, soil EDTA-washing showed the greatest reduction of bacterial community tolerance to Cu and Ni, respectively, while for Zn the soil amendment with PB was the most effective remediation technique. Thus, bacterial community tolerance to heavy metals successfully detect differences in the effectiveness of the three remediation techniques. PB Agronomy SN 20734395 YR 2022 FD 2022-09-23 LK http://hdl.handle.net/11093/3896 UL http://hdl.handle.net/11093/3896 LA eng NO Agronomy, 12(10): 2280 (2022) NO Ministerio de Economía y Competitividad | Ref. CTM2015-73422-JIN DS Investigo RD 19-ene-2025