RT Journal Article
T1 In silico and functional analyses of immunomodulatory peptides encrypted in the human gut metaproteome
A1 Cambeiro Pérez, Noelia
A1 Hidalgo Cantabrana, Claudio
A1 Moro García, Marco Antonio
A1 Blanco Míguez, Aitor
A1 Fernández Riverola, Florentino
A1 Riestra, Sabino
A1 GARCIA LOURENÇO, Analia Maria
A1 Alonso Arias, Rebeca
A1 Margolles, Abelardo
A1 Martínez Carballo, Elena
A1 Sanchez Garcia, Borja
K1 2414 Microbiología
K1 2304.18 Polipéptidos y Proteínas
AB This work supports the massive presence of potential immunomodulatory peptides in the human gut metaproteome. These peptides were identified through the MAHMI database as potentially anti-inflammatory, and sixteen of them synthesized for characterize their mechanism of action. From them, peptide HM14 was encrypted in an extracellular protein produced by Bifidobacterium longum, a common member of the human microbiota, and displayed the highest anti-inflammatory capability. Molecular mechanism of action of HM14 pointed to a specific interaction between this immunomodulatory peptide and antigen presenting cells, which resulted in a higher formation of iTreg cells. Moreover, HM14 was effective in decreasing pro-inflammatory parameters in PBMCs isolated from a cohort of Crohn's patients. Finally, non-targeted metabolomics confirmed the ability of HM14 to modulate the metabolic activity of PBMCs to fulfil its energy and biosynthetic requirements. Overall, our combined in silico/multiomics approach supports the human gut metaproteome as a source for immunomodulatory peptides.
PB Journal of Functional Foods
SN 17564646
YR 2020
FD 2020-07
LK http://hdl.handle.net/11093/3893
UL http://hdl.handle.net/11093/3893
LA eng
NO Journal of Functional Foods, 70: 103969 (2020)
NO Ministerio de Economía y Competitividad | Ref. AGL2013-44761-P
DS Investigo
RD 13-ene-2025