RT Journal Article T1 Development of an active contour based algorithm to perform the segmentation of soot agglomerates in uneven illumination TEM imaging A1 Paz Penín, Maria Concepcion A1 Cabarcos Rey, Adrián A1 Vence Fernandez, Jesús A1 Gil Pereira, Christian K1 2205.04 Mecánica de Fluidos K1 2204.04 Mecánica de Fluidos AB Automatic methods for morphological characterisation of nano-particulate from microscopy images usually involve the use of thresholding procedures, which cannot effectively deal with inhomogeneous backgrounds. In this paper, an algorithm based on an active contour model is proposed to extract soot projections from uneven illumination TEM imaging. The presented method involves a pre-processing stage where a median filter and a size normalisation step are performed. Then, the model applied is based on a level set formulation with local region fitting for noisy image segmentation. Once the active model reaches a convergence result, soot regions are isolated and labelled even under inhomogeneous illuminations. The accuracy of the proposed algorithm was evaluated by statistical comparisons of soot size descriptors extracted from 80 testing images, with results obtained by the application of the Otsu's segmentation method and manual measurements. Excellent agreement was found for uneven illumination micrographs without compromising the performance on regular imaging. PB Powder Technology SN 00325910 YR 2022 FD 2022-03 LK http://hdl.handle.net/11093/3765 UL http://hdl.handle.net/11093/3765 LA eng NO Powder Technology, 400, 117260 (2022) NO Agencia Estatal de Investigación | Ref. ENE2017-87855-R DS Investigo RD 03-dic-2024