RT Journal Article
T1 Identification of candidate host serum and saliva biomarkers for a better diagnosis of active and latent tuberculosis infection
A1 Estévez Martínez, Olivia
A1 Anibarro Garcia, Luis
A1 Garet, Elina
A1 Pallares, Ángeles
A1 Pena Rodríguez, Alberto
A1 Villaverde Taboada, Carlos Manuel Ramon
A1 del Campo, Víctor
A1 González Fernández, Maria Africa
K1 3205.08 Enfermedades Pulmonares
K1 3205.05 Enfermedades Infecciosas
K1 2412 Inmunología
AB In our work, we aim to identify new candidate host biomarkers to discriminate between active TB patients (n = 28), latent infection (LTBI; n = 27) and uninfected (NoTBI; n = 42) individuals. For that, active TB patients and their contacts were recruited that donated serum and saliva samples. A multiplex assay was performed to study the concentration of different cytokines, chemokines and growth factors. Proteins with significant differences between groups were selected and logistic regression and the area under the ROC curve (AUC) was used to assess the diagnostic accuracy. The best marker combinations that discriminate active TB from NoTBI contacts were [IP-10 + IL-7] in serum and [Fractalkine + IP-10 + IL-1α + VEGF] in saliva. Best discrimination between active TB and LTBI was achieved using [IP-10 + BCA-1] in serum (AUC = 0.83) and IP-10 in saliva (p = 0.0007; AUC = 0.78). The levels of TNFα (p = 0.003; AUC = 0.73) in serum and the combination of [Fractalkine+IL-12p40] (AUC = 0.83) in saliva, were able to differentiate between NoTBI and LTBI contacts. In conclusion, different individual and combined protein markers could help to discriminate between active TB and both uninfected and latently-infected contacts. The most promising ones include [IP-10 + IL-7], [IP-10 + BCA-1] and TNFα in serum and [Fractalkine + IP-10 + IL-1α + VEGF], IP-10 and [Fractalkine+IL-12p40] in saliva.
PB PLoS ONE
SN 19326203
YR 2020
FD 2020-07-20
LK http://hdl.handle.net/11093/3687
UL http://hdl.handle.net/11093/3687
LA eng
NO PLoS ONE, 15(7): e0235859 (2020)
NO Xunta de Galicia | Ref. ED431C 2016/041
DS Investigo
RD 23-ene-2025