RT Journal Article T1 3,3′,4,5′-Tetramethoxy-trans-stilbene improves insulin resistance by activating the IRS/PI3K/Akt pathway and inhibiting oxidative stress A1 Tan, Yi A1 Miao, Lingchao A1 Xiao, Jianbo A1 Cheang, Wai San K1 2302.14 Glúcidos K1 2302 Bioquímica K1 3309.03 Antioxidantes en Los Alimentos AB The potential anti-diabetic effect of resveratrol derivative, 3,3′,4,5′-tetramethoxy-trans-stilbene (3,3′,4,5′-TMS) and its underlying mechanism in high glucose (HG) and dexamethasone (DXMS)-stimulated insulin-resistant HepG2 cells (IR-HepG2) were investigated. 3,3′,4,5′-TMS did not reduce the cell viability of IR-HepG2 cells at the concentrations of 0.5–10 µM. 3,3′,4,5′-TMS increased the potential of glucose consumption and glycogen synthesis in a concentration-dependent manner in IR-HepG2 cells. 3,3′,4,5′-TMS ameliorated insulin resistance by enhancing the phosphorylation of glycogen synthase kinase 3 beta (GSK3β), inhibiting phosphorylation of insulin receptor substrate-1 (IRS-1), and activating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway in IR-HepG2 cells. Furthermore, 3,3′,4,5′-TMS significantly suppressed levels of reactive oxygen species (ROS) with up-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) expression. To conclude, the beneficial effect of 3,3′,4,5′-TMS against insulin resistance to increase glucose consumption and glycogen synthesis was mediated through activation of IRS/PI3K/Akt signaling pathways in the IR-HepG2 cells, accomplished with anti-oxidative activity through up-regulation of Nrf2. PB Current Issues In Molecular Biology SN 14673037 YR 2022 FD 2022-05-12 LK http://hdl.handle.net/11093/3599 UL http://hdl.handle.net/11093/3599 LA eng NO Current Issues In Molecular Biology, 44(5): 2175-2185 (2022) NO University of Macau | Ref. SRG2019-00154-ICMS DS Investigo RD 03-nov-2024