RT Journal Article T1 In search of antioxidant peptides from porcine liver hydrolysates using analytical and peptidomic approach A1 López Pedrouso, María A1 Lorenzo Rodríguez, José Manuel A1 Borrajo Bastero, Paula A1 Franco, Daniel K1 2302.24 Péptidos K1 2302 Bioquímica K1 3309.21 Alimentos Proteínicos AB The search for antioxidant peptides as health-promoting agents is of great scientific interest for their biotechnological applications. Thus, the main goal of this study was to identify antioxidant peptides from pork liver using alcalase, bromelain, flavourzyme, and papain enzymes. All liver hydrolysates proved to be of adequate quality regarding the ratio EAA/NEAA, particularly flavourzyme hydrolysates. The peptidomic profiles were significantly different for each enzyme and their characterizations were performed, resulting in forty-four differentially abundant peptides among the four treatments. Porcine liver hydrolysates from alcalase and bromelain are demonstrated to have the most antioxidant capacity. On the other hand, hydrophobic amino acid residues (serine, threonine, histidine and aspartic acid) might be reducing the hydrolysates antioxidant capacity. Seventeen peptides from collagen, albumin, globin domain-containing protein, cytochrome β, fructose-bisphosphate aldolase, dihydropyrimidinase, argininosuccinate synthase, and ATP synthase seem to be antioxidant. Further studies are necessary to isolate these peptides and test them in in vivo experiments. PB Antioxidants SN 20763921 YR 2021 FD 2021-12-24 LK http://hdl.handle.net/11093/3530 UL http://hdl.handle.net/11093/3530 LA eng NO Antioxidants, 11(1): 27 (2021) NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria | Ref. RTA 2017-00024- CO4-04 DS Investigo RD 04-dic-2024