RT Journal Article
T1 Central regulation of food intake is not affected by inclusion of defatted Tenebrio molitor larvae meal in diets for European sea bass (Dicentrarchus labrax)
A1 Basto, Ana
A1 Valente, Luisa M.P.
A1 Conde Sieira, Marta
A1 Soengas Fernández, José Luis
K1 2401.13 Fisiología Animal
K1 2401.14-4 Taxonomía Animal. Peces
K1 2302.19 Procesos Metabólicos
AB The homeostatic regulation of food intake in fish has been thoroughly studied in the last years with dietary nutrient composition is one of the factors involved. Despite several studies addressed the impact of insect meal-based diets in food intake of fish, there is no knowledge about their impact in mechanisms of food intake regulation. This study aimed to explore underlying mechanisms through evaluation of dietary fishmeal (FM) replacement by defatted (d-) Tenebrio molitor (dTM) larvae meal in European sea bass. Fish (55 ± 2 g) fed a FM-based diet (CTRL) were compared with those fed two  experimental diets containing increasing levels of dTM to replace 40 and 80% of FM (TM40 and TM80, respectively). After ten weeks of feeding, fish food intake was calculated; samples of plasma and different brain areas (hypothalamus and telencephalon) were collected at different post-prandial times (2, 6 and 24 h after feeding) to evaluate circulating metabolites and mRNA abundance in hypothalamus and  telencephalon of neuropeptides involved in food intake regulation. No differences occurred in food intake and weight gain among diets. Plasma cholesterol levels decreased 24 h after feeding in fish fed TM40; fish fed dTM diets had higher cholesterol levels, 2 and 6 h after feeding, than those fed CTRL diet. Increased non-esterified fatty acids (NEFA) levels occurred in plasma of fish fed TM80, regardless of the sampling time. At central level, no changes occurred in the mRNA abundance of neuropeptide Y (npy), agouti-related protein 2 (agrp2), pro-opio melanocortin a (pomca) or cocaine- and amphetamine-related transcript 2 (cartpt2). The obtained results suggest that dietary FM replacement by dTM up to 80% in European sea bass does not affect food intake or its central homeostatic regulation, supporting the use of dTM as FM replacement in diets for European sea bass.
PB Aquaculture
SN 00448486
YR 2021
FD 2021-11-15
LK http://hdl.handle.net/11093/2321
UL http://hdl.handle.net/11093/2321
LA eng
NO Aquaculture, 544: 737088 (2021)
NO Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG
NO Agencia Estatal de Investigación | Ref. PID2019-103969RB-C31
DS Investigo
RD 03-dic-2024