RT Journal Article
T1 Liquid chromatography coupled to high-resolution mass spectrometry for the confirmation of Caribbean Ciguatoxin-1 as the main toxin responsible for Ciguatera poisoning caused by fish from European Atlantic coasts
A1 Estevez Bastos, Pablo
A1 Sibat, Manoella
A1 Leao Martins, José Manuel
A1 Reis Costa, Pedro
A1 Gago Martínez, Ana Maria Consuelo
A1 Hess, Philipp
K1 2301.03 Análisis Cromatográfico
K1 3206.11 Toxicidad de Los Alimentos
AB Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal).
PB Toxins
SN 20726651
YR 2020
FD 2020-04-21
LK http://hdl.handle.net/11093/1833
UL http://hdl.handle.net/11093/1833
LA eng
NO Toxins, 12(4): 267 (2020)
NO European Food Safety Authority | Ref. GP/EFSA/AFSCO/2015/03
DS Investigo
RD 04-dic-2024