RT Journal Article T1 Exploring the use of Bryophyllum as natural source of bioactive compounds with antioxidant activity to prevent lipid oxidation of fish oil-In-water emulsions A1 García Pérez, Pascual A1 Losada Barreiro, Sonia A1 Bravo Díaz, Carlos Daniel A1 Gallego Veigas, Pedro Pablo K1 2210.03 Cinética Química K1 2210.08 Emulsiones K1 3309.03 Antioxidantes en Los Alimentos AB The current industrial requirements for food naturalness are forcing the development of new strategies to achieve the production of healthier foods by replacing the use of synthetic additives with bioactive compounds from natural sources. Here, we investigate the use of plant tissue culture as a biotechnological solution to produce plant-derived bioactive compounds with antioxidant activity and their application to protect fish oil-in-water emulsions against lipid peroxidation. The total phenolic content of Bryophyllum plant extracts ranges from 3.4 to 5.9 mM, expressed as gallic acid equivalents (GAE). The addition of Bryophyllum extracts to 4:6 fish oil-in-water emulsions results in a sharp (eight-fold) increase in the antioxidant efficiency due to the incorporation of polyphenols to the interfacial region. In the emulsions, the antioxidant efficiency of extracts increased linearly with concentration and levelled off at 500 μM GAE, reaching a plateau region. The antioxidant efficiency increases modestly (12%) upon increasing the pH from 3.0 to 5.0, while an increase in temperature from 10 to 30 °C causes a six-fold decrease in the antioxidant efficiency. Overall, results show that Bryophyllum plant-derived extracts are promising sources of bioactive compounds with antioxidant activity that can be eventually be used to control lipid oxidation in food emulsions containing (poly)unsaturated fatty acids. PB Plants SN 22237747 YR 2020 FD 2020-08-11 LK http://hdl.handle.net/11093/1731 UL http://hdl.handle.net/11093/1731 LA eng NO Plants, 9(8): 1012 (2020) NO Xunta de Galicia | Ref. ED431D 2017/18 DS Investigo RD 28-abr-2025