RT Dissertation/Thesis
T1 Detection and characterization of photosynthetic pigments in marine phytoplankton
T2 Detección y caracterización de pigmentos fotosintéticos en fitoplancton marino
A1 Gavalás Olea, Antonio
K1 2303.23 Química de Los Pigmentos
K1 2510.01 Oceanografía Biológica
K1 2302.25 Fotosíntesis
AB Fractions of nano- and picoplankton are important components of the phytoplankton in the oceans. These organisms, like the rest of the marine phototrophs, present a complex set of photosynthetic pigments (chlorophylls, carotenoids and phycobilins) that, selectively distributed in different taxa of eukaryotes and bacteria, play a physiological role which reaches a global importance. The determination of pigments in oceanic waters has to face several difficulties, resulting in analytical needs repeatedly emphasized:(1) the description of the marine ecosystem need large number of samples; (2) the samples may contain variety of species with pigment endowments of great complexity which complicate their analysis (3) in extensive oceanic áreas photosynthetic organisms occur in very low cell densities, compromising pigment detection with the currently available techniques and (4) many pigments of high biological significance are difficult to ascertain due to analytical interferences with structurally similar ones. The need for the best techniques to describe populations of the phytoplankton of oligotrophic ecosystems and the knowledge of the analytical possibilities of new techniques lead us to propose the following objectives:1. To develop methodologies for the preconcentration of pigment extracts, aimed at increasing the sensitivity of the analysis. 2. Design new chromatographic methods with innovative selectivities, able to discriminate a greater number of pigments and, therefore, increase the capacity of classification and distinction among taxonomic entities in phytoplankton populations.3. Obtain rapid chromatographic systems, with analytical times in all cases less than 15 minutes.4. Characterization the structures of new chlorophylls and carotenoids (chemotaxonomic biomarkers) mainly of picoplankton species. 5. Study, using all the methodologies previously designed, the pigment distribution of populations of phytoplankton in the South of the Mediterranean Sea.
YR 2018
FD 2018-11-14
LK http://hdl.handle.net/11093/1082
UL http://hdl.handle.net/11093/1082
LA eng
NO Ministerio de Economía y Competitividad de España. Proyecto OLIGOPIGMENTS | Ref. CTM2012-32181
DS Investigo
RD 05-dic-2024