Show simple item record

dc.contributor.authorMoyano, Ana
dc.contributor.authorBlanch Lombarte, Oscar
dc.contributor.authorTarancón Diez, Laura
dc.contributor.authorPedreño Lopez, Nuria
dc.contributor.authorArenas Busto, Miguel 
dc.contributor.authorAlvaro, Tamara
dc.contributor.authorCasado, Concepción
dc.contributor.authorOlivares, Isabel
dc.contributor.authorVera, Mar
dc.contributor.authorRodríguez, Carmen
dc.contributor.authordel Romero, Jorge
dc.contributor.authorLópez Galíndez, Cecilio
dc.contributor.authorRuiz Mateos, Ezequiel
dc.contributor.authorPrado, Julia G.
dc.contributor.authorPernas, María
dc.date.accessioned2024-03-22T11:51:07Z
dc.date.available2024-03-22T11:51:07Z
dc.date.issued2022-03-26
dc.identifier.citationRetrovirology, 19: 6 (2022)spa
dc.identifier.issn17424690
dc.identifier.urihttp://hdl.handle.net/11093/6492
dc.description.abstractBackground Long-Term Non-Progressors (LTNPs) are untreated Human Immunodeficiency virus type 1 (HIV-1) infected individuals able to control disease progression for prolonged periods. However, the LTNPs status is temporary, as viral load increases followed by decreases in CD4 + T-cell counts. Control of HIV-1 infection in LTNPs viremic controllers, have been associated with effective immunodominant HIV-1 Gag-CD8 + T-cell responses restricted by protective HLA-B alleles. Individuals carrying HLA-B*14:02 control HIV-1 infection is related to an immunodominant Env-CD8 + T-cell response. Limited data are available on the contribution of HLA-B*14:02 CD8 + T -cells in LTNPs. Results In this study, we performed a virological and immunological detailed analysis of an HLA-B*14:02 LNTP individual that lost viral control (LVC) 27 years after HIV-1 diagnosis. We analysed viral evolution and immune escape in HLA-B*14:02 restricted CD8 + T -cell epitopes and identified viral evolution at the Env-EL9 epitope selecting the L592R mutation. By IFN-γ ELISpot and immune phenotype, we characterized HLA- B*14:02 HIV-1 CD8 + T cell responses targeting, Gag-DA9 and Env-EL9 epitopes before and after LVC. We observed an immunodominant response against the Env-EL9 epitope and a decreased of the CD8 T + cell response over time with LVC. Loss of Env-EL9 responses was concomitant with selecting K588R + L592R mutations at Env-EL9. Finally, we evaluated the impact of Env-EL9 escape mutations on HIV-1 infectivity and Env protein structure. The K588R + L592R escape variant was directly related to HIV-1 increase replicative capacity and stability of Env at the LVC. Conclusions These findings support the contribution of immunodominant Env-EL9 CD8 + T-cell responses and the imposition of immune escape variants with higher replicative capacity associated with LVC in this LNTP. These data highlight the importance of Env-EL9 specific-CD8 + T-cell responses restricted by the HLA-B*14:02 and brings new insights into understanding long-term HIV-1 control mediated by Env mediated CD8 + T-cell responses.en
dc.description.sponsorshipInstituto de Salud Carlos III | Ref. PI13/02269spa
dc.description.sponsorshipInstituto de Salud Carlos III | Ref. PI17/00164spa
dc.description.sponsorshipInstituto de Salud Carlos III | Ref. PI16/0684spa
dc.description.sponsorshipInstituto de Salud Carlos III | Ref. PI19/01127spa
dc.description.sponsorshipMinisterio de Economía y Competitividad | Ref. RD12/0017/0028spa
dc.description.sponsorshipMinisterio de Economía y Competitividad | Ref. RD16/0025/0020spa
dc.description.sponsorshipGeneralitat de Catalunya | Ref. AGAUR-FI_B 00582spa
dc.description.sponsorshipAgencia Estatal de Investigación | Ref. RYC-2015-18241spa
dc.description.sponsorshipAgencia Estatal de Investigación | Ref. PID2019-107931GA-I00spa
dc.description.sponsorshipXunta de Galicia | Ref. ED431F 2018/08spa
dc.description.sponsorshipMinisterio de Economía y Competitividad | Ref. RD16/0025/0041spa
dc.language.isoengspa
dc.publisherRetrovirologyspa
dc.relationinfo:eu-repo/grantAgreement/ISCIII//PI13%2F02269/ES
dc.relationinfo:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI17%2F00164
dc.relationinfo:eu-repo/grantAgreement/ISCIII//PI16%2F0684/ES
dc.relationinfo:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI19%2F01127
dc.relationinfo:eu-repo/grantAgreement/MINECO//RD12%2F0017%2F0028/ES
dc.relationinfo:eu-repo/grantAgreement/MINECO//RD16%2F0025%2F0020/ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107931GA-I00/ES
dc.relationinfo:eu-repo/grantAgreement/MINECO//RD16%2F0025%2F0041/ES
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleImmunoescape of HIV-1 in Env-EL9 CD8 + T cell response restricted by HLA-B*14:02 in a Non progressor who lost twenty-seven years of HIV-1 controlen
dc.typearticlespa
dc.rights.accessRightsopenAccessspa
dc.identifier.doi10.1186/s12977-022-00591-7
dc.identifier.editorhttps://retrovirology.biomedcentral.com/articles/10.1186/s12977-022-00591-7spa
dc.publisher.departamentoBioquímica, xenética e inmunoloxíaspa
dc.publisher.grupoinvestigacionXenómica e Biomedicinaspa
dc.subject.unesco2409 Genéticaspa
dc.subject.unesco2403 Bioquímicaspa
dc.subject.unesco2420 Virologíaspa
dc.date.updated2024-02-19T12:42:38Z
dc.computerCitationpub_title=Retrovirology|volume=19|journal_number=|start_pag=6|end_pag=spa


Files in this item

[PDF]

    Show simple item record

    Attribution 4.0 International
    Except where otherwise noted, this item's license is described as Attribution 4.0 International