dc.contributor.author | Moyano, Ana | |
dc.contributor.author | Blanch Lombarte, Oscar | |
dc.contributor.author | Tarancón Diez, Laura | |
dc.contributor.author | Pedreño Lopez, Nuria | |
dc.contributor.author | Arenas Busto, Miguel | |
dc.contributor.author | Alvaro, Tamara | |
dc.contributor.author | Casado, Concepción | |
dc.contributor.author | Olivares, Isabel | |
dc.contributor.author | Vera, Mar | |
dc.contributor.author | Rodríguez, Carmen | |
dc.contributor.author | del Romero, Jorge | |
dc.contributor.author | López Galíndez, Cecilio | |
dc.contributor.author | Ruiz Mateos, Ezequiel | |
dc.contributor.author | Prado, Julia G. | |
dc.contributor.author | Pernas, María | |
dc.date.accessioned | 2024-03-22T11:51:07Z | |
dc.date.available | 2024-03-22T11:51:07Z | |
dc.date.issued | 2022-03-26 | |
dc.identifier.citation | Retrovirology, 19: 6 (2022) | spa |
dc.identifier.issn | 17424690 | |
dc.identifier.uri | http://hdl.handle.net/11093/6492 | |
dc.description.abstract | Background Long-Term Non-Progressors (LTNPs) are untreated Human Immunodeficiency virus type 1 (HIV-1) infected individuals able to control disease progression for prolonged periods. However, the LTNPs status is temporary, as viral load increases followed by decreases in CD4 + T-cell counts. Control of HIV-1 infection in LTNPs viremic controllers, have been associated with effective immunodominant HIV-1 Gag-CD8 + T-cell responses restricted by protective HLA-B alleles. Individuals carrying HLA-B*14:02 control HIV-1 infection is related to an immunodominant Env-CD8 + T-cell response. Limited data are available on the contribution of HLA-B*14:02 CD8 + T -cells in LTNPs. Results In this study, we performed a virological and immunological detailed analysis of an HLA-B*14:02 LNTP individual that lost viral control (LVC) 27 years after HIV-1 diagnosis. We analysed viral evolution and immune escape in HLA-B*14:02 restricted CD8 + T -cell epitopes and identified viral evolution at the Env-EL9 epitope selecting the L592R mutation. By IFN-γ ELISpot and immune phenotype, we characterized HLA- B*14:02 HIV-1 CD8 + T cell responses targeting, Gag-DA9 and Env-EL9 epitopes before and after LVC. We observed an immunodominant response against the Env-EL9 epitope and a decreased of the CD8 T + cell response over time with LVC. Loss of Env-EL9 responses was concomitant with selecting K588R + L592R mutations at Env-EL9. Finally, we evaluated the impact of Env-EL9 escape mutations on HIV-1 infectivity and Env protein structure. The K588R + L592R escape variant was directly related to HIV-1 increase replicative capacity and stability of Env at the LVC. Conclusions These findings support the contribution of immunodominant Env-EL9 CD8 + T-cell responses and the imposition of immune escape variants with higher replicative capacity associated with LVC in this LNTP. These data highlight the importance of Env-EL9 specific-CD8 + T-cell responses restricted by the HLA-B*14:02 and brings new insights into understanding long-term HIV-1 control mediated by Env mediated CD8 + T-cell responses. | en |
dc.description.sponsorship | Instituto de Salud Carlos III | Ref. PI13/02269 | spa |
dc.description.sponsorship | Instituto de Salud Carlos III | Ref. PI17/00164 | spa |
dc.description.sponsorship | Instituto de Salud Carlos III | Ref. PI16/0684 | spa |
dc.description.sponsorship | Instituto de Salud Carlos III | Ref. PI19/01127 | spa |
dc.description.sponsorship | Ministerio de Economía y Competitividad | Ref. RD12/0017/0028 | spa |
dc.description.sponsorship | Ministerio de Economía y Competitividad | Ref. RD16/0025/0020 | spa |
dc.description.sponsorship | Generalitat de Catalunya | Ref. AGAUR-FI_B 00582 | spa |
dc.description.sponsorship | Agencia Estatal de Investigación | Ref. RYC-2015-18241 | spa |
dc.description.sponsorship | Agencia Estatal de Investigación | Ref. PID2019-107931GA-I00 | spa |
dc.description.sponsorship | Xunta de Galicia | Ref. ED431F 2018/08 | spa |
dc.description.sponsorship | Ministerio de Economía y Competitividad | Ref. RD16/0025/0041 | spa |
dc.language.iso | eng | spa |
dc.publisher | Retrovirology | spa |
dc.relation | info:eu-repo/grantAgreement/ISCIII//PI13%2F02269/ES | |
dc.relation | info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI17%2F00164 | |
dc.relation | info:eu-repo/grantAgreement/ISCIII//PI16%2F0684/ES | |
dc.relation | info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI19%2F01127 | |
dc.relation | info:eu-repo/grantAgreement/MINECO//RD12%2F0017%2F0028/ES | |
dc.relation | info:eu-repo/grantAgreement/MINECO//RD16%2F0025%2F0020/ES | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107931GA-I00/ES | |
dc.relation | info:eu-repo/grantAgreement/MINECO//RD16%2F0025%2F0041/ES | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Immunoescape of HIV-1 in Env-EL9 CD8 + T cell response restricted by HLA-B*14:02 in a Non progressor who lost twenty-seven years of HIV-1 control | en |
dc.type | article | spa |
dc.rights.accessRights | openAccess | spa |
dc.identifier.doi | 10.1186/s12977-022-00591-7 | |
dc.identifier.editor | https://retrovirology.biomedcentral.com/articles/10.1186/s12977-022-00591-7 | spa |
dc.publisher.departamento | Bioquímica, xenética e inmunoloxía | spa |
dc.publisher.grupoinvestigacion | Xenómica e Biomedicina | spa |
dc.subject.unesco | 2409 Genética | spa |
dc.subject.unesco | 2403 Bioquímica | spa |
dc.subject.unesco | 2420 Virología | spa |
dc.date.updated | 2024-02-19T12:42:38Z | |
dc.computerCitation | pub_title=Retrovirology|volume=19|journal_number=|start_pag=6|end_pag= | spa |