Show simple item record

dc.contributor.authorCeinos Caride, Rosa María 
dc.contributor.authorFrigato, Elena
dc.contributor.authorPagano, Cristina
dc.contributor.authorFröhlich, Nadine
dc.contributor.authorNegrini, Pietro
dc.contributor.authorCavallari, Nicola
dc.contributor.authorVallone, Daniela
dc.contributor.authorFuselli, Silvia
dc.contributor.authorBertolucci, Cristiano
dc.contributor.authorFoulkes, Nicholas S
dc.date.accessioned2022-12-27T11:26:00Z
dc.date.available2022-12-27T11:26:00Z
dc.date.issued2018-06-08
dc.identifier.citationScientific Reports, 8(1): 8754 (2018)spa
dc.identifier.issn20452322
dc.identifier.urihttp://hdl.handle.net/11093/4299
dc.description.abstractLight represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors.en
dc.description.sponsorshipUniversidad de Ferrara | Ref. FAR2014-2017spa
dc.language.isoengspa
dc.publisherScientific Reportsspa
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleMutations in blind cavefish target the light-regulated circadian clock gene, period 2en
dc.typearticlespa
dc.rights.accessRightsopenAccessspa
dc.identifier.doi10.1038/s41598-018-27080-2
dc.identifier.editorhttps://www.nature.com/articles/s41598-018-27080-2spa
dc.subject.unesco2401.13 Fisiología Animalspa
dc.subject.unesco2411 Fisiología Humanaspa
dc.subject.unesco2409 Genéticaspa
dc.date.updated2022-12-27T09:47:45Z
dc.computerCitationpub_title=Scientific Reports|volume=8|journal_number=1|start_pag=8754|end_pag=spa


Files in this item

[PDF]

    Show simple item record

    Attribution 4.0 International
    Except where otherwise noted, this item's license is described as Attribution 4.0 International