Optimization of a new selective pressurized liquid extraction methodology for determining organic pollutants in wild boar livers

Abstract

In this study, a new selective pressurised liquid extraction (SPLE) methodology was optimised for determining about 70 organic pollutants (OPs) including organochlorine (OCPs), organophosphate (OPPs) and pyrethroid (PYRs) pesticides, polychlorinated biphenyls (PCBs), polybromodiphenyl ethers (PBDEs), as well as, polycyclic aromatic hydrocarbons (PAHs) in wild boar liver samples considering the temperature, pressure and time of contact between the solvent and the matrix as influential variables. Clean-up of extracts was performed by solid-phase extraction (SPE) using EZ-POP cartridges. Detection of OPs was carried out by gas chromatography (GC) coupled to tandem mass spectrometry (QqQ-MS/MS). This new approach offers: A new non-time consuming SPLE methodology for determining about 70 OPs in wild boar. Recoveries achieved ranged between 74 to 119 % with RSD less than 20 %. Detection and quantification limits in the low to mid pg/g range.